High Fidelity PCR Reagents and Kits
Invitrogen™ Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity
Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.
Thermo Scientific™ Phusion U Hot Start DNA Polymerases
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
Thermo Scientific™ Phusion U Multiplex PCR Master Mixes
Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.
Thermo Scientific™ Phusion Site-Directed Mutagenesis Kit
Generate mutations with this fast and reliable system.
Invitrogen™ AccuPrime™ Pfx SuperMix
AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.
Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase & dNTP Mix (10 mM each)
This package includes Phusion High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).
Invitrogen™ Platinum™ SuperFi™ U Multiplex Master Mix
Invitrogen Platinum SuperFi U Multiplex Master Mix is a product designed to synthesize multiple custom amplicons for further use in next-generation sequencing.
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase & dNTP Mix (10 mM each)
This package includes Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).
G-Biosciences High Fidelity DNA Polymerase 2X Master Mix
High Fidelity DNA Polymerase 2x Master Mix is used for PCR, Cloning, long and high GC content template DNA amplification and Diagnostics purposes.
Takara Bio CloneAmp™ HiFi PCR Premix, 200 Rxns
A convenient 2X PCR master mix that is optimized for high-fidelity and fast PCR. It is designed for use with Takara's In-Fusion Cloning. CloneAmp HiFi PCR Premix is suitable for high-throughput cloning applications. 200 Rxns
New England Biolabs, Inc. NEBNext® High-Fidelity 2X PCR Master Mix – 50 reactions
NEBNext High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.
- Next generation sequencing library amplification
- High fidelity PCR
- High-throughput PCR
Supply Solutions Roche - Expand HiFi PCR Sys., 100 u; pkg of 2,500 U (11759078001 [10x250 U]) pkg of 100 U (11732641001) pkg of 500 U (11732650001 [2x250 U])
Roche - Expand HiFi PCR Sys., 100 u; pkg of 2,500 U (11759078001 [10x250 U]) pkg of 100 U (11732641001) pkg of 500 U (11732650001 [2x250 U])
New England Biolabs, Inc. Q5® Hot Start High-Fidelity 2X Master Mix – 100 rxn (2 x 1.25 ml)
The Q5 Hot Start High-Fidelity 2X Master Mix features a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity 2X Master Mix is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 Hot Start High-Fidelity Master Mix contains 2 mM Mg++. Q5 Hot Start High-Fidelity 2X Master Mix is unlike typical, lower fidelity PCR enzymes.
New England Biolabs, Inc. Q5® High-Fidelity 2X Master Mix – 100 reactions (50 µl vol)
The Q5 High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format. The Q5 High- Fidelity 2X Master Mix features a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM Mg++. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.